Phenol bioultra, for molecular biology, tesaturated, 73% t synonym. To the best of our knowledge, the information contained herein is accurate. Cool tanks drums with water spray and remove them to safety. Safety data sheet according to 29cfr19101200 and ghs rev. Phenol is poorly soluble in water and, at room temperature, forms only a 6. State regulations california proposition 65 this product does not contain any proposition 65 chemicals. I know i should just add phenol to the water until it no longer goes into solution but i want a ballpark ratio of phenol to water. Determining the ph of water saturatedphenol solutions at severn biotech we use a ph probe to check phenol, which needs to be carefully rinsed after each measurement. Preparation of buffer saturated phenol for rna extractions. Call a physician or poison control center immediately. You can taste and smell phenol at levels lower than those that are associated with harmful effects. The sodium salt of phenol, sodium phenoxide, is far more water soluble. Phenol is a colorless or white solid when it is pure. Bp1751i400 synonyms buffered phenol solution recommended use laboratory chemicals 2.
A high density shower is preferable to reduce phenol uptake. Special exposure closed containers exposed to heat may explode. Product and company identification product name phenol, saturated ph 4. Use water spray to keep fireexposed containers cool. Phenol is both a manufactured chemical and a natural substance. The ph of phenol solutions is difficult to measure directly, although the ph of the buffer solution above the phenol is a good indication.
The fume hood in our lab is filled with equipment so i cant. Store the unused portion at 2 to 8c under nitrogen gas. Causes severe skin burns and eye damage toxic if inhaled suspected of causing genetic defects may cause damage to organs through prolonged or. Accidental release measures personal precautions use personal protective equipment as required. Phenol is an organic compound appreciably soluble in water, with about 84. Firstaid measures general advice show this safety data sheet to the doctor in attendance. In case of eye contact rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. To prepare activated charcoal slurry, mix thoroughly 50 g of activated charcoal in 400 ml about 2 cups water. How to make water saturated phenol for rna extraction. Do not use a straight stream of water, which will scatter and spread the fire. Photocatalytic oxidation of benzene to phenol using. Add 8hydroxyquinoline to a final concentration of 0.
Extinguishing use water spray, fog, foam, dry chemical, co or other agents as. Phenol saturated for molecular biology company name. Immediately flush eyes with excess water for 15 minutes, lifting lower and upper eyelids occasionally. Making watersaturated phenol dec072006 hello all, this is a pretty silly question but how do i make watersaturated phenol from phenol crystals. Material safety data sheet phenol tris saturated sol. Acid phenol to solid phenol add rnasefree water until there is a layer of water on top of the phenol. Material safety data sheet phenol saturated for molecular biology section 1 chemical product and company identification msds name. Preparation of phenol for use in molecular biology applications is a timeconsuming and o. Test the ph periodically and do not use if the ph is water saturated phenol for rna extraction. Phenol bioultra, for molecular biology, tesaturated, 73%. Use water spray, alcoholresistant foam, dry chemical or carbon dioxide. Phenol is moderately soluble in water about 8 g of phenol will dissolve in 100 g of water.
First aid measures always seek professional medical attention after first aid measures are provided. If you try to dissolve more than this, you get two layers of liquid. Homogeneous mixtures of phenol and water at phenol to water mass ratios of 2. The top layer is a solution of phenol in water, and the bottom one a solution of water in phenol. Is there any method to prepare water saturated acidic phenol for rna extraction. The fume hood in our lab is filled with equipment so i cant move in both the stirplate and weigh machine at the same time. Cardinal gracious road, chakala, andheri east, mumbai 400 099. Determining the ph of water saturatedphenol solutions. Dec 07, 2006 this is a pretty silly question but how do i make water saturated phenol from phenol crystals. Phenol united states environmental protection agency. Phenol material safety data sheet cas no 108952 sdsmsds.
The halflives for phenol biodegradation in 15 river water samples averaged 4 and 7 days at 30 and 20c, respectively, but only about 20% 0. Phenol liquid and vapor pose a severe health hazard when inhaled or exposed to bare skin. Toxic and hazardous industrial chemicals safety manual. One more, the water saturated phenol will used for rna extraction. This safety data sheet has been prepared in accordance with the globally harmonized system for the classification and labeling of chemicals ghs. Add enough deionized water to fill 100 g bottle to the base of the neck. Phenol is used primarily in the production of phenolic resins and in the manufacture of nylon and other synthetic fibers. Is there any method to prepare water saturated acidic. The addition of 8hydroxyquinoline increases stability and colors the phenol yellow, so that the separation of the aqueous and organic phases is facilitated. I needed to prepare water saturated phenol for rna extraction. The information provided in this material safety data sheet represents a compilation of data drawn directly from various sources available to us. A bottle of buffer is included for most dna applications, that require a higher ph of 7.
Phenol saturated for molecular biology section 1 chemical product and company identification msds name. Fire fighting water should be contained if possible, as it is toxic and can cause environmental damage. Please check the following link and pdf attachment. It is usually prepared by the hospital pharmacy because it is not commercially available in premixed liquid form. Phenol is a benzene ring with one hydroxyl group substituted for a hydrogen atom. Test the ph periodically and do not use if the ph is phenol will be lost during the preparation of the buffer saturated phenol. To measure the ph of the phenol directly, ambion recommends mixing 2 ml of water saturated phenol with 5 ml of methanol and ml water to form a uniform solution, which can be measured with a ph meter. In case of skin contact wash off with soap and plenty of water. Phenol standard, ppm persistence and degradability not established. Phenol has a distinct odor that is sickeningly sweet and tarry. Material safety data sheet west liberty university. Never give anything by mouth to an unconscious person. Preparation of buffer saturated phenol for dna extraction.
The buffer saturated phenol may be stored at 4c for 1 month for dna extraction. Rinse thoroughly with plenty of water for at least 15 minutes and. Sigmaaldrich offers a number of phenol solution products. Ingest immediately about 350 ml 5 mlkg body weight of activated charcoal slurry.
Jul 01, 2014 information on phenol also known as carbolic acid and hydroxybenzene, for responding to chemical incidents. Determining the ph of water saturated phenol solutions at severn biotech we use a ph probe to check phenol, which needs to be carefully rinsed after each measurement. None of the chemicals in this product are considered highly hazardous by osha. Lesser amounts of water will merely dilute the phenol and expand the area of exposure. We do use ph paper for quick spot checks on small volumes outlined below are two alternative methods for measuring ph of phenol. Thermo scientific pierce phenol, saturated is phenol supplied as a saturated solution ph 6. Material is lighter than water, and so a fire can be spread by the use of water. Cap securely and mix the organic and aqueous phases until they form a fine emulsion. Persistence and degradability picric acid, saturated persistence and degradability not established.
General description this phenol solution is a clear liquid saturated with citrate buffer. Wear full protective equipment, including suitable respiratory protection. Use water to cool nearby containers and structures. Picric acid, wetted 88 89 1 persistence and degradability not readily biodegradable in water. All information recommendations and suggestions appearing herein concerning this product are based upon data obtained from the manufacturer andor recognized technical sources. Phenol solutions can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol chloroform protocols. Phenol evaporates more slowly than water, and a moderate amount can form a solution with water. Material safety data sheet bromine water, saturated. Ambion water saturated phenol is supplied premixed and at ph 6. A bottle of buffer is included for most dna applications, that. Physical properties of phenol chemistry libretexts.
Water saturated phenol is used in rna and dna extraction procedures and is provided in one bottle of 400 ml. Molecular biology grade for nucleic acid purifications. Product and company identification phenol synonymsgeneral names. Material safety data sheet phenol section 1 chemical product and company identification msds name. Picric acid, wetted 88 89 1 ec50 other aquatic organisms 1 240 mgl algae. Molecular biology grade for nucleic acid purifications product description. No component of this product present at levels greater than or equal to 0. Product and company identification phenol solution, 5% synonymsgeneral names. Phenol biodegradation by microorganisms is also influenced by temperature and microbe counts. Making watersaturated phenol general lab techniques. Read label before use obtain special instructions before use do not handle until all safety precautions have been read and understood.
Peg300 or peg400, rather than water, for immediate first aid treatment of dermal. Next the polysomes are dissociated with sds and proteinase k and finally the protein is removed by several ph. Identification of the substancemixture and of the companyundertaking. Vapors can travel to a source of ignition and flash back. It is the users responsibility to determine the safety, toxicity and suitability. In case of fire, use water, dry chemical, chemical foam, or alcoholresistant foam. Phenol 108952 persistence and degradability readily biodegradable in water. Sigmaaldrich material safety data sheet date printed. During fire, irritating and toxic gases of hydrogen chloride may be.
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